6W5Y
Inferred receptor binding domain of human endogenous retrovirus envelope EnvP(b)1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9998 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 243.310, 37.350, 66.990 |
Unit cell angles | 90.00, 102.28, 90.00 |
Refinement procedure
Resolution | 49.561 - 2.500 |
R-factor | 0.2237 |
Rwork | 0.222 |
R-free | 0.25380 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.988 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.561 | 2.589 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.127 | 0.886 |
Rmeas | 0.142 | 0.993 |
Rpim | 0.063 | 0.442 |
Number of reflections | 20893 | 2023 |
<I/σ(I)> | 12.65 | 1.83 |
Completeness [%] | 99.8 | 99.75 |
Redundancy | 4.9 | 5 |
CC(1/2) | 0.996 | 0.752 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291.15 | 0.5 M Sodium chloride, 0.1 M Bis- tris(hydroxymethyl)aminomethane propane pH 7.0, 20 %(w/v) polyethylene glycol 4000 |