6W5U
NPC1 structure in GDN micelles at pH 5.5, conformation b
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.9 |
Software | RELION,Gctf |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |