6W5B
N124D Deamidation Mutant of Human gammaD-Crystallin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.8856 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.649, 51.493, 93.794 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.170 - 1.150 |
R-factor | 0.152 |
Rwork | 0.151 |
R-free | 0.17220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hk0 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.214 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.170 | 1.191 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.068 | 0.519 |
Rmeas | 0.071 | 0.575 |
Rpim | 0.020 | 0.241 |
Number of reflections | 58473 | 5481 |
<I/σ(I)> | 17.43 | 2.2 |
Completeness [%] | 99.3 | 93.9 |
Redundancy | 11.6 | 5.5 |
CC(1/2) | 0.999 | 0.866 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M ammonium chloride, 20% w/v PEG 3350 |