6W54
Crystal Structure of Gallic Acid Decarboxylase from Arxula adeninivorans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | H 3 |
Unit cell lengths | 81.971, 81.971, 103.055 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.700 - 1.500 |
R-factor | 0.1414 |
Rwork | 0.140 |
R-free | 0.16620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Unpublished |
RMSD bond length | 0.008 |
RMSD bond angle | 0.981 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmeas | 0.094 | 0.357 |
Rpim | 0.044 | 0.236 |
Number of reflections | 36574 | 689 |
<I/σ(I)> | 43 | 5.8 |
Completeness [%] | 88.5 | 33.8 |
Redundancy | 4.1 | 2 |
CC(1/2) | 0.950 | 0.819 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | PEG 3350, Potassium Formate, MES |