6W4Y
Structure of full-length human lambda-6A light chain JTO in complex with hydantoin stabilizer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-09-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.976484 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.713, 82.341, 95.912 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.650 - 1.910 |
| R-factor | 0.1832 |
| Rwork | 0.181 |
| R-free | 0.23220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mg4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.586 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.650 | 2.010 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rpim | 0.025 | 0.025 |
| Number of reflections | 39919 | 5702 |
| <I/σ(I)> | 22.7 | |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 8 | 8.1 |
| CC(1/2) | 1.000 | 0.920 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 296 | 20% PEG 3350 and 0.25 M NH4H2PO4 at 23 degrees C |
