6W4B

The crystal structure of Nsp9 RNA binding protein of SARS CoV-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97919
Spacegroup name	P 61 2 2
Unit cell lengths	89.883, 89.883, 132.587
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.940 - 2.950
R-factor	0.2417
Rwork	0.240
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uw7
RMSD bond length	0.002
RMSD bond angle	0.697
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.190	1.753
Rmeas	0.199	1.874
Rpim	0.058	0.646
Number of reflections	7299	275
<I/σ(I)>	17.4	1.09
Completeness [%]	97.1	79
Redundancy	12	6.9
CC(1/2)	0.987	0.452

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	289	1.8 M di-Ammonium hydrogen citrate, 0.1 M Sodium acetate