6W3X
Crystal structure of ligand-binding domain of Campylobacter jejuni chemoreceptor Tlp3 in complex with L-valine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-07 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.570, 137.540, 49.100 |
| Unit cell angles | 90.00, 94.49, 90.00 |
Refinement procedure
| Resolution | 28.120 - 1.400 |
| R-factor | 0.1147 |
| Rwork | 0.113 |
| R-free | 0.15430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xmr |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.642 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.180 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.047 | 0.308 |
| Number of reflections | 103884 | 103884 |
| <I/σ(I)> | 11.4 | 3.1 |
| Completeness [%] | 94.3 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 3350, sodium citrate and ammonium sulphate |
