6W3T

Crystal structure of ligand-binding domain of Campylobacter jejuni chemoreceptor Tlp3 in complex with L-norvaline

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-10
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	41.931, 137.770, 49.190
Unit cell angles	90.00, 93.92, 90.00

Refinement procedure

Resolution	39.969 - 2.100
R-factor	0.1613
Rwork	0.159
R-free	0.20420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xmr
RMSD bond length	0.006
RMSD bond angle	0.794
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.230	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.077
Rmeas		0.313
Number of reflections	31594
<I/σ(I)>	10.6
Completeness [%]	97.6
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 3350, sodium citrate and ammonium sulphate