6W3T
Crystal structure of ligand-binding domain of Campylobacter jejuni chemoreceptor Tlp3 in complex with L-norvaline
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-10 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.931, 137.770, 49.190 |
Unit cell angles | 90.00, 93.92, 90.00 |
Refinement procedure
Resolution | 39.969 - 2.100 |
R-factor | 0.1613 |
Rwork | 0.159 |
R-free | 0.20420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xmr |
RMSD bond length | 0.006 |
RMSD bond angle | 0.794 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.230 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.077 | |
Rmeas | 0.313 | |
Number of reflections | 31594 | |
<I/σ(I)> | 10.6 | |
Completeness [%] | 97.6 | |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 3350, sodium citrate and ammonium sulphate |