6W3R
Crystal structure of ligand-binding domain of Campylobacter jejuni chemoreceptor Tlp3 in complex with 3-methylisoleucine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-02-08 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.260, 138.080, 48.830 |
| Unit cell angles | 90.00, 93.82, 90.00 |
Refinement procedure
| Resolution | 35.990 - 1.380 |
| R-factor | 0.1234 |
| Rwork | 0.120 |
| R-free | 0.17980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xmr |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.477 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.990 | 1.460 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.068 | 0.342 |
| Number of reflections | 91649 | |
| <I/σ(I)> | 9.1 | 2.6 |
| Completeness [%] | 81.1 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 3350, sodium citrate and ammonium sulphate |
