6W36
Crystal structure of FAM46C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.674, 58.951, 173.035 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.447 - 2.854 |
R-factor | 0.2194 |
Rwork | 0.215 |
R-free | 0.25910 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.474 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.850 | 2.850 |
Rpim | 0.023 | |
Number of reflections | 12832 | 609 |
<I/σ(I)> | 39.4 | |
Completeness [%] | 99.2 | 95 |
Redundancy | 6.8 | |
CC(1/2) | 0.850 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 5.5 | 293 | 1 M ammonium sulfate, 0.1 M Bis-Tris, pH 5.5, 1% PEG3350 |