6W36
Crystal structure of FAM46C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.674, 58.951, 173.035 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.447 - 2.854 |
| R-factor | 0.2194 |
| Rwork | 0.215 |
| R-free | 0.25910 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.474 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rpim | 0.023 | |
| Number of reflections | 12832 | 609 |
| <I/σ(I)> | 39.4 | |
| Completeness [%] | 99.2 | 95 |
| Redundancy | 6.8 | |
| CC(1/2) | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 5.5 | 293 | 1 M ammonium sulfate, 0.1 M Bis-Tris, pH 5.5, 1% PEG3350 |
