6W2P
APE1 endonuclease product complex L104R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-23 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.387, 61.780, 72.278 |
| Unit cell angles | 83.71, 78.56, 88.02 |
Refinement procedure
| Resolution | 24.450 - 1.940 |
| R-factor | 0.2021 |
| Rwork | 0.201 |
| R-free | 0.23760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dff |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.991 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.980 |
| High resolution limit [Å] | 1.940 | 1.950 |
| Rmeas | 0.074 | 0.815 |
| Number of reflections | 99515 | 4296 |
| <I/σ(I)> | 19.54 | 2.01 |
| Completeness [%] | 99.4 | |
| Redundancy | 4.3 | |
| CC(1/2) | 0.770 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 10% PEG20000, 100 mM sodium citrate, pH 5.0, and 200 mM MgCl2 |
