6W1W
Crystal Structure of Motility Associated Killing Factor B from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-27 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 137.718, 137.718, 233.834 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 66.050 - 2.580 |
| R-factor | 0.2769 |
| Rwork | 0.276 |
| R-free | 0.28580 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.522 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.050 | 2.620 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.139 | 0.953 |
| Number of reflections | 81344 | 40719 |
| <I/σ(I)> | 7.3 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.7 | 10.1 |
| CC(1/2) | 0.992 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M ammonium acetate, 0.1 M HEPES-NaOH, pH 7.5, 25% w/v PEG3350 |
