6W1F

Crystal structure of Streptococcus thermophilus SHP pheromone receptor Rgg3 bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 65
Unit cell lengths	90.790, 90.790, 245.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.395 - 3.200
R-factor	0.2217
Rwork	0.217
R-free	0.26620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yv6
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.260
High resolution limit [Å]	3.200	8.670	3.200
Rmerge	0.054	0.031	0.664
Rmeas	0.057	0.032	0.699
Rpim	0.018	0.010	0.216
Number of reflections	18799	968	928
<I/σ(I)>	8
Completeness [%]	99.5	98.9	99.9
Redundancy	10.3	10.2	10.3
CC(1/2)	0.986	0.997	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	298	170 mM ammonium acetate, 85 mM sodium citrate, 22% PEG 4,000, and 15% glycerol