6W1B
N-terminal domain of mouse surfactant protein B with bound lipid, Y59A/H79A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-17 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.335, 169.527, 73.714 |
Unit cell angles | 90.00, 126.90, 90.00 |
Refinement procedure
Resolution | 84.760 - 2.310 |
R-factor | 0.2422 |
Rwork | 0.241 |
R-free | 0.26190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6vyn lipid removed |
RMSD bond length | 0.002 |
RMSD bond angle | 0.672 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.760 | 2.397 |
High resolution limit [Å] | 2.310 | 2.310 |
Rmerge | 0.084 | 2.030 |
Number of reflections | 37983 | 3077 |
<I/σ(I)> | 13.34 | 0.72 |
Completeness [%] | 97.5 | 80.13 |
Redundancy | 6.9 | 6 |
CC(1/2) | 0.995 | 0.518 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293.15 | 100 mM Na/K phosphate pH 6.2, 200 mM NaCl, 47.5% (w/v) PEG 200 |