6W1A

Crystal structure of Streptococcus dysgalactiae SHP pheromone receptor Rgg2 bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-05
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.18076
Spacegroup name	P 65
Unit cell lengths	110.269, 110.269, 173.984
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.570 - 2.800
R-factor	0.2356
Rwork	0.234
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yv6
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.800	7.590	2.800
Rmerge	0.102	0.038	1.518
Rmeas	0.109	0.041	1.619
Rpim	0.038	0.014	0.563
Number of reflections	29582	1507	1468
<I/σ(I)>	10.5
Completeness [%]	99.9	99	100
Redundancy	8.3	8.1	8.3
CC(1/2)	0.999	0.999	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	100 mM trilithium citrate and 24% PEG 3,350