6W11

The structure of Sulfolobus solfataricus Csa3 in complex with cyclic tetraadenylate (cA4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	160
Detector technology	IMAGE PLATE
Collection date	2019-06-13
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	46.094, 93.369, 105.191
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.910 - 2.460
R-factor	0.2067
Rwork	0.203
R-free	0.24280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wte
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.450	6.650	2.450
Rmerge	0.079	0.049	0.820
Rmeas	0.084	0.052	0.863
Rpim	0.026	0.017	0.265
Total number of observations	171587
Number of reflections	16551	933	824
<I/σ(I)>	23.9
Completeness [%]	96.8	97.7	100
Redundancy	10.4	9.7	10.4
CC(1/2)		0.998	0.737

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	14-22% PEG MME 550, 100mM imidazole, 150mM malate, 10mM cA4