6W0L

Henipavirus W protein interacts with 14-3-3 to modulate host gene expression

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9357
Spacegroup name	C 2 2 21
Unit cell lengths	83.040, 112.000, 62.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.968 - 2.300
R-factor	0.2128
Rwork	0.210
R-free	0.26470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dat
Data reduction software	MOSFLM
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.970	24.970	2.380
High resolution limit [Å]	2.300	8.910	2.300
Rmerge	0.188	0.068	0.698
Rmeas	0.212	0.077	0.793
Rpim	0.096	0.036	0.370
Total number of observations	63050	1139	5697
Number of reflections	13246	249	1277
<I/σ(I)>	5.2	10.4	2.1
Completeness [%]	99.7	94.5	99.8
Redundancy	4.8	4.6	4.5
CC(1/2)	0.978	0.988	0.344

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277.15	31% PEG 400, 0.1 M HEPES pH 7.5, 0.2 M CaCl2 and 2 mM DTT