6W0J
Closed-gate KcsA incubated in BaCl2/NaCl
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 98 |
Detector technology | PIXEL |
Collection date | 2018-12-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | I 4 |
Unit cell lengths | 156.329, 156.329, 76.359 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 68.612 - 2.500 |
R-factor | 0.1914 |
Rwork | 0.190 |
R-free | 0.21840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k4c |
RMSD bond length | 0.008 |
RMSD bond angle | 0.957 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.612 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 32029 | 3625 |
<I/σ(I)> | 7.1 | |
Completeness [%] | 99.9 | |
Redundancy | 9.6 | |
CC(1/2) | 0.990 | 0.550 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5 |