6W0I

Closed-gate KcsA soaked in 10mM KCl/5mM BaCl2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	98
Detector technology	PIXEL
Collection date	2018-10-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	I 4
Unit cell lengths	154.238, 154.238, 75.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.971 - 2.328
R-factor	0.2019
Rwork	0.200
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k4c
RMSD bond length	0.008
RMSD bond angle	0.953
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.971	2.400
High resolution limit [Å]	2.300	2.300
Number of reflections	37609	3385
<I/σ(I)>	12
Completeness [%]	98.6
Redundancy	10.6
CC(1/2)	0.920	0.290

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5