6W0C

Open-gate KcsA soaked in 4 mM BaCl2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	98
Detector technology	PIXEL
Collection date	2017-02-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	I 4
Unit cell lengths	155.970, 155.970, 74.316
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.144 - 3.556
R-factor	0.2026
Rwork	0.200
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k4c
RMSD bond length	0.004
RMSD bond angle	0.775
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.144	3.600
High resolution limit [Å]	3.500	3.500
Rmerge	0.220
Number of reflections	10740	2414
<I/σ(I)>	6.5
Completeness [%]	98.5
Redundancy	4.9
CC(1/2)	0.980	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5