6VZF
Crystal Structure of Atg11 Coiled-Coil 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-11-02 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979100 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 103.105, 103.105, 37.520 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.610 - 2.030 |
R-factor | 0.2273 |
Rwork | 0.226 |
R-free | 0.24590 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.719 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.3) |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.650 | 44.650 | 2.080 |
High resolution limit [Å] | 2.030 | 9.080 | 2.030 |
Rmerge | 0.107 | 0.059 | 1.852 |
Rmeas | 0.109 | 0.060 | 1.901 |
Rpim | 0.024 | 0.014 | 0.427 |
Total number of observations | 303942 | 3292 | 21698 |
Number of reflections | 15063 | 189 | 1110 |
<I/σ(I)> | 16.7 | 57.6 | 2 |
Completeness [%] | 99.9 | 97.7 | 100 |
Redundancy | 20.2 | 17.4 | 19.5 |
CC(1/2) | 0.999 | 0.999 | 0.661 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 90 mM LiNaK, 0.1 M Buffer system 4 pH 6.5 (MOPSO + Bis Tris), 50% v/v precipitant mix 6 (25% w/v PEG 4000, 40% w/v 1,2,6-hexanetriol) | |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 90 mM LiNaK, 0.1 M Buffer system 4 pH 6.5 (MOPSO + Bis Tris), 50% v/v precipitant mix 6 (25% w/v PEG 4000, 40% w/v 1,2,6-hexanetriol) |