6VZE

C-terminal domain of mouse surfactant protein B crystallized at low pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	52.193, 79.008, 71.917
Unit cell angles	90.00, 106.43, 90.00

Refinement procedure

Resolution	68.980 - 1.900
R-factor	0.2117
Rwork	0.210
R-free	0.23980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vz0 residues A26-V45
RMSD bond length	0.004
RMSD bond angle	0.740
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.980	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.106	1.102
Number of reflections	43545	4071
<I/σ(I)>	9.54	1.09
Completeness [%]	97.4	93.14
Redundancy	3.5	3.5
CC(1/2)	0.991	0.211

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293.15	170 mM ammonium sulfate, 21-23% PEG 4000, 15% glycerol