6VZD
N-terminal domain of mouse surfactant protein B (K46E/R51E mutant) with bound lipid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.793, 66.124, 59.620 |
Unit cell angles | 90.00, 97.37, 90.00 |
Refinement procedure
Resolution | 59.130 - 1.880 |
R-factor | 0.1727 |
Rwork | 0.170 |
R-free | 0.21750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6VYN (lipid removed) |
RMSD bond length | 0.006 |
RMSD bond angle | 0.736 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.130 | 1.947 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.100 | 1.511 |
Number of reflections | 31237 | 2186 |
<I/σ(I)> | 40.86 | 2.41 |
Completeness [%] | 92.2 | |
Redundancy | 53.8 | |
CC(1/2) | 1.000 | 0.897 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293.15 | 100 mM citrate buffer pH 5.4, 23-25% PEG 4000, 14-15% 2-propanol |