6VY4
Crystal structure of Hendra receptor binding protein head domain in complex with human neutralizing antibody HENV-32
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-06-26 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97856 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 195.889, 84.490, 122.834 |
Unit cell angles | 90.00, 95.93, 90.00 |
Refinement procedure
Resolution | 49.506 - 2.000 |
R-factor | 0.1853 |
Rwork | 0.183 |
R-free | 0.22610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vsm |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.510 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.088 | 0.743 |
Number of reflections | 134705 | 19582 |
<I/σ(I)> | 11.7 | |
Completeness [%] | 100.0 | |
Redundancy | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.5 M ammonium sulfate, 5% isopropanol |