6VY0
SthK P300A cyclic nucleotide-gated potassium channel in a putative active state, in complex with cAMP
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 0.001 |
Energy Filter | GIF Bioquantum |
Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 6.68 |
Number of Particle | 657943 |
Software | RELION,Leginon,CTFFIND |
Symmetry | C4 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |