6VWW
Crystal Structure of NSP15 Endoribonuclease from SARS CoV-2.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-19 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 63 |
Unit cell lengths | 150.539, 150.539, 111.310 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.060 - 2.200 |
R-factor | 0.1612 |
Rwork | 0.158 |
R-free | 0.17770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDBID 2H85 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.433 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.174 | 0.924 |
Number of reflections | 71461 | 3176 |
<I/σ(I)> | 10 | 1.07 |
Completeness [%] | 98.5 | 88.3 |
Redundancy | 5.1 | 2.6 |
CC(1/2) | 0.967 | 0.358 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M Calcium Acetate, 0.1 M HEPES pH 7.5, 10 %(w/v) PEG8000 |