6VWA

C-terminal regulatory domain of the chloride transporter KCC-1 from C. elegans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99
Spacegroup name	P 32 1 2
Unit cell lengths	75.647, 75.647, 146.865
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.955 - 2.196
R-factor	0.1838
Rwork	0.182
R-free	0.22260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vw9
RMSD bond length	0.007
RMSD bond angle	0.896
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.196	2.196
Number of reflections	24878	2448
<I/σ(I)>	10.4	0.51
Completeness [%]	99.9	99.71
Redundancy	5.4	5.4
CC(1/2)	0.995	0.476

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.1 M HEPES pH 7.5, 50% w/v PEG 200