6VW9
C-terminal regulatory domain of the chloride transporter KCC-1 from C. elegans, proteolyzed during crystallization
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97910 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.320, 65.546, 56.523 |
| Unit cell angles | 90.00, 97.17, 90.00 |
Refinement procedure
| Resolution | 40.790 - 1.800 |
| R-factor | 0.1843 |
| Rwork | 0.183 |
| R-free | 0.21450 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.114 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.790 | 1.860 |
| High resolution limit [Å] | 1.796 | 1.796 |
| Number of reflections | 34639 | 29973 |
| <I/σ(I)> | 13.83 | 0.73 |
| Completeness [%] | 97.9 | 91.01 |
| Redundancy | 9.4 | 9.4 |
| CC(1/2) | 0.999 | 0.454 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.1 M TRIS pH 8.5, 5% w/v PEG 8000, 20% w/v PEG 300, 10% glycerol |
