6VW8
Formate Dehydrogenase FdsABG subcomplex FdsBG from C. necator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 193 |
Detector technology | PIXEL |
Collection date | 2018-11-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 132.656, 127.050, 99.840 |
Unit cell angles | 90.00, 123.50, 90.00 |
Refinement procedure
Resolution | 48.534 - 2.300 |
R-factor | 0.183 |
Rwork | 0.182 |
R-free | 0.22310 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.056 |
Data reduction software | DIALS |
Data scaling software | Aimless (0.7.4) |
Phasing software | CRANK2 |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.534 | 48.530 | 2.360 |
High resolution limit [Å] | 2.300 | 10.290 | 2.300 |
Rmerge | 0.129 | 0.066 | 0.462 |
Rmeas | 0.141 | 0.072 | 0.514 |
Rpim | 0.056 | 0.029 | 0.223 |
Total number of observations | 4225 | 23427 | |
Number of reflections | 61209 | 710 | 4497 |
<I/σ(I)> | 8.9 | 17.7 | 3.3 |
Completeness [%] | 99.9 | 99 | 99.5 |
Redundancy | 6.1 | 6 | 5.2 |
CC(1/2) | 0.985 | 0.994 | 0.915 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 2-propanol, ammonium sulfate, HEPES-NaOH, in an anaerobic chamber |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 2-propanol, ammonium sulfate, HEPES-NaOH, in an anaerobic chamber |