6VW7
Formate Dehydrogenase FdsABG subcomplex FdsBG from C. necator - NADH bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2019-12-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000010 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.656, 127.050, 99.840 |
| Unit cell angles | 90.00, 123.50, 90.00 |
Refinement procedure
| Resolution | 48.534 - 2.000 |
| R-factor | 0.1598 |
| Rwork | 0.159 |
| R-free | 0.19910 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.968 |
| Data reduction software | HKL-3000 |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.560 | 48.560 | 2.030 |
| High resolution limit [Å] | 2.000 | 10.950 | 2.000 |
| Rmerge | 0.064 | 0.039 | 0.426 |
| Rmeas | 0.072 | 0.043 | 0.474 |
| Rpim | 0.031 | 0.018 | 0.207 |
| Total number of observations | 475014 | 3027 | 24341 |
| Number of reflections | 93063 | 590 | 4607 |
| <I/σ(I)> | 15.4 | 39.4 | 3.9 |
| Completeness [%] | 99.9 | 98.8 | 100 |
| Redundancy | 5.1 | 5.1 | 5.3 |
| CC(1/2) | 0.999 | 0.998 | 0.936 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 287 | 2-propanol, ammonium sulfate, HEPES-NaOH, in an anaerobic chamber |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 287 | 2-propanol, ammonium sulfate, HEPES-NaOH, in an anaerobic chamber |
