6VVW

W0 fused 4-OT wild type symmetric trimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0332
Spacegroup name	P 3
Unit cell lengths	83.866, 83.866, 149.604
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.930 - 2.100
Rwork	0.275
R-free	0.33350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5unq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (v1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.930	2.170
High resolution limit [Å]	2.100	2.100
Number of reflections	68347	6690
<I/σ(I)>	10.9
Completeness [%]	99.5	97.1
Redundancy	5.4
CC(1/2)	0.992	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298.15	0.2 M ammonium sulfate, 27-37% PEG5000 MME