6VVG
Structure of the Cydia pomonella Granulovirus kinase, PK-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.937, 115.370, 119.395 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.570 - 2.010 |
| R-factor | 0.1946 |
| Rwork | 0.193 |
| R-free | 0.22480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v70 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.648 |
| Data reduction software | XDS |
| Data scaling software | Aimless (7.0) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 41.940 | 41.940 | 2.060 |
| High resolution limit [Å] | 2.010 | 8.990 | 2.010 |
| Rmerge | 0.294 | 0.057 | 1.307 |
| Rmeas | 0.306 | 0.061 | 1.395 |
| Rpim | 0.085 | 0.019 | 0.477 |
| Total number of observations | 486841 | 5809 | 18803 |
| Number of reflections | 38430 | 530 | 2276 |
| <I/σ(I)> | 9.6 | 29.8 | 1.6 |
| Completeness [%] | 97.3 | 99.5 | 80.6 |
| Redundancy | 12.7 | 11 | 8.3 |
| CC(1/2) | 0.993 | 0.998 | 0.554 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 300 mM Ammonium chloride, 16.5 % (w/v) PEG 3350 and 1 mM AMP |
