6VUP
Reverse Transcriptase Diabody with R83T/E85C Mutations
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 72.942, 86.686, 37.078 |
Unit cell angles | 90.00, 116.23, 90.00 |
Refinement procedure
Resolution | 43.340 - 1.400 |
R-factor | 0.1332 |
Rwork | 0.131 |
R-free | 0.17060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6vrp |
RMSD bond length | 0.007 |
RMSD bond angle | 0.927 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.041 | 0.049 |
Rmeas | 0.049 | 0.301 |
Number of reflections | 39530 | 1905 |
<I/σ(I)> | 24.7 | |
Completeness [%] | 97.4 | |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.4 | 293 | 6 % v/v 2-Propanol, 0.1 M Sodium Acetate trihydrate pH 4.5, 26% PEG 550 MME |