6VUO
Reverse Transcriptase Diabody with S82bC/R83T Mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X17B1 |
| Synchrotron site | NSLS |
| Beamline | X17B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.372, 55.195, 161.867 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.556 - 1.449 |
| R-factor | 0.1418 |
| Rwork | 0.141 |
| R-free | 0.17210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6vrp |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.511 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.449 | 1.450 |
| Rmerge | 0.055 | 0.656 |
| Rmeas | 0.057 | 0.718 |
| Number of reflections | 124175 | 8188 |
| <I/σ(I)> | 54.5 | |
| Completeness [%] | 99.7 | |
| Redundancy | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | 14% PEG 1000, 0.1 M Sodium Citrate pH 5.7 |
