6VUD

Crystal structure of a ribosome recycling factor from Ehrlichia chaffeensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2020-02-06
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	C 1 2 1
Unit cell lengths	79.660, 93.230, 73.420
Unit cell angles	90.00, 109.73, 90.00

Refinement procedure

Resolution	38.920 - 2.350
R-factor	0.194
Rwork	0.190
R-free	0.23460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	top 8 models from HHPred search: 4KDD_A 1DD5_A 1IS1_A 5MLC_9 1EH1_A 4GFQ_A 6ERI_Az 1GE9_A
RMSD bond length	0.006
RMSD bond angle	0.659
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MR-Rosetta
Refinement software	PHENIX (1.18.rc1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.410
High resolution limit [Å]	2.350	10.510	2.350
Rmerge	0.031	0.023	0.575
Rmeas	0.036	0.027	0.656
Number of reflections	21055	227	1529
<I/σ(I)>	23.94	55.3	2.95
Completeness [%]	99.6	91.2	99.9
Redundancy	4.23	3.511	4.279
CC(1/2)	0.999	0.998	0.882

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	290	RigakuReagents JCSG+ screen, condition C2: 20% (w/V) PEG 6000, 1000mM Lithium chloride, 100mM sodium citrate tribasic / citric acid pH 4.2 EhchA.00948.a.AE1.PS38617 at 13.45 mg/ml: cryo: 20% EG, tray 314079c2: puck wzi8-8