6VU7
Crystal structure of YbjN, a putative transcription regulator from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2018-03-09 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.462, 110.891, 59.924 |
Unit cell angles | 90.00, 116.71, 90.00 |
Refinement procedure
Resolution | 29.930 - 2.590 |
R-factor | 0.2403 |
Rwork | 0.237 |
R-free | 0.29460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fr7 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.596 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15_3448) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.640 |
High resolution limit [Å] | 2.590 | 2.600 |
Rmerge | 0.126 | 0.597 |
Rpim | 0.083 | 0.536 |
Number of reflections | 19643 | 961 |
<I/σ(I)> | 13.59 | 2.68 |
Completeness [%] | 99.7 | |
Redundancy | 3.2 | |
CC(1/2) | 0.536 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 298 | 0.1 M diammionium citrate, 0.1 M MES pH 5.2, 26% (w/v) PEG3350, 2% isopropanol |