6VTS

Crystal structure of G16S human Galectin-7 mutant in complex with lactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	30.300, 76.870, 113.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.730 - 1.900
R-factor	0.1817
Rwork	0.179
R-free	0.22140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gal
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.730	45.740	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.067	0.033	1.224
Rmeas	0.070	0.034	1.277
Number of reflections	21749	2353	2116
<I/σ(I)>	23.5	59.7	2.4
Completeness [%]	99.9	99.83	99.86
Redundancy	12.58		12.41
CC(1/2)	1.000	1.000	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295.15	0.1 M Tris pH 8, 0.1 M NaCl, 16 % PEG 3350, 150 mM alpha-Lactose