6VTQ
Crystal structure of G16C human Galectin-7 mutant in complex with lactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-28 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.370, 66.210, 111.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.660 - 1.950 |
R-factor | 0.1898 |
Rwork | 0.185 |
R-free | 0.24450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gal |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.7.12) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.660 | 42.670 | 2.020 |
High resolution limit [Å] | 1.950 | 4.200 | 1.950 |
Rmerge | 0.107 | 0.048 | 1.037 |
Rmeas | 0.112 | 0.050 | 1.081 |
Number of reflections | 17138 | 1860 | 1704 |
<I/σ(I)> | 16.7 | 39.6 | 3.1 |
Completeness [%] | 100.0 | 99.95 | 100 |
Redundancy | 12.59 | 11.68 | 12.66 |
CC(1/2) | 0.999 | 0.999 | 0.868 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295.15 | 0.1 M Tris pH 8, 0.1 M Sodium Chloride, 25 % PEG3350, 7.5 mM alpha-lactose |