6VSX

X-ray crystal structure of the C-terminal domain of Bacillus subtilis RNA polymerase binding helicase HelD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-02
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5414
Spacegroup name	C 1 2 1
Unit cell lengths	106.963, 38.813, 44.427
Unit cell angles	90.00, 101.45, 90.00

Refinement procedure

Resolution	24.730 - 2.000
R-factor	0.1752
Rwork	0.172
R-free	0.20150
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.802
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.730	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.044	0.206
Number of reflections	11905	463
<I/σ(I)>	60.1
Completeness [%]	96.7	72
Redundancy	20
CC(1/2)	0.991	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.2	300	0.1M Na/K phosphate, pH 6.2, 0.2 M NaCl, 50 % PEG200