6VSU
Arginase from Arabidopsis thaliana in Complex with Ornithine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | I 41 |
| Unit cell lengths | 267.322, 267.322, 262.894 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.970 - 2.250 |
| R-factor | 0.1594 |
| Rwork | 0.159 |
| R-free | 0.19680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6vss |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.783 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.064 | 0.483 |
| Number of reflections | 429624 | 43258 |
| <I/σ(I)> | 17.1 | 2.5 |
| Completeness [%] | 99.0 | 99.9 |
| Redundancy | 3.3 | 3.4 |
| CC(1/2) | 0.994 | 0.747 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 50 mM L-arginine, 50 mM L-glutamic acid, 22% PEG Smear Broad, 5% glycerol |
