6VSR

Crystal structure of macaque anti-HIV-1 antibody RM20F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	61.459, 75.646, 115.068
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.721 - 2.176
R-factor	0.1992
Rwork	0.197
R-free	0.24090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5csz
RMSD bond length	0.002
RMSD bond angle	0.551
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.730	2.240
High resolution limit [Å]	2.176	2.200
Number of reflections	28053	1364
<I/σ(I)>	7.7
Completeness [%]	98.6
Redundancy	4
CC(1/2)	0.840	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	The RM20F Fab was crystallized from a solution containing 10 mg/mL protein in 1X TBS with a well solution containing 0.1M MES, pH 5.0 and 2M ammonium sulfate. The crystals were cryoprotected by soaking in a well solution supplemented with 30% ethylene glycol