6VSL

Crystal structure of a human fucosylated IgG1 Fc expressed in tobacco plants (Nicotiana benthamiana)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920
Spacegroup name	P 21 21 21
Unit cell lengths	49.860, 79.920, 138.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.910 - 2.100
R-factor	0.2096
Rwork	0.207
R-free	0.24990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ave
RMSD bond length	0.010
RMSD bond angle	1.102
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.210
High resolution limit [Å]	2.100	2.100
Rpim	0.061	0.479
Number of reflections	32561	4712
<I/σ(I)>	5.4	1
Completeness [%]	98.7	99.3
Redundancy	3.3	3.4
CC(1/2)	0.990	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	15% PEG4000, 0.1 M HEPES, pH 7.0