6VSH
Crystal structure of apo Dicamba Monooxygenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-15 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 |
| Unit cell lengths | 80.170, 80.170, 159.346 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.260 - 3.000 |
| R-factor | 0.1979 |
| Rwork | 0.193 |
| R-free | 0.25170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gte |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.278 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | CNX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 45738 | 4598 |
| <I/σ(I)> | 25.8 | 6.69 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 8.1 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 10-20 mg/mL protein in 25 mM Tris-HCl, pH 8.0, 10 mM sodium chloride, 0.1 mM EDTA, trace PMSF + 1:1 reservoir solution (15-20% w/v PEG6000, 100 mM sodium citrate, pH 6.0) in 2-6 uL droplet |
