6VRP
Reverse Transcriptase Diabody with R83T Mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-05 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.728, 55.460, 157.304 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.800 - 1.600 |
R-factor | 0.1474 |
Rwork | 0.146 |
R-free | 0.18270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4x4x |
RMSD bond length | 0.004 |
RMSD bond angle | 0.751 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.046 | 0.368 |
Rmeas | 0.050 | 0.398 |
Number of reflections | 61848 | 3029 |
<I/σ(I)> | 39.14 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.6 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2 M Ammonium Sulfate, 20% PEG 3350 |