6VRM
T cell receptor-p53-HLA-A2 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-29 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 89.405, 120.608, 119.693 |
Unit cell angles | 90.00, 108.38, 90.00 |
Refinement procedure
Resolution | 19.790 - 2.610 |
R-factor | 0.1949 |
Rwork | 0.191 |
R-free | 0.27530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d2l 3sjv 2ak4 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.752 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.900 | 2.700 |
High resolution limit [Å] | 2.610 | 2.610 |
Rmerge | 0.044 | 0.343 |
Number of reflections | 36118 | 3641 |
<I/σ(I)> | 12.2 | |
Completeness [%] | 98.4 | |
Redundancy | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 295 | 20% (w/v) polyacrylic acid 5100, 0.1 M HEPES, 20 mM MgCl2 |