6VR8
Structure of a pseudomurein peptide ligase type E from Methanothermus fervidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-18 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 61 |
| Unit cell lengths | 119.369, 119.369, 71.088 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.070 - 1.900 |
| R-factor | 0.1874 |
| Rwork | 0.185 |
| R-free | 0.23280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.758 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.800 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.940 |
| Rmerge | 0.102 | |
| Number of reflections | 45561 | 2895 |
| <I/σ(I)> | 14.6 | 4.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 22 | |
| CC(1/2) | 0.997 | 0.884 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 293 | 0.2 M ammonium sulfate, 30% w/v PEG4000 |
