6VR1
Complex of HLA-A2, a class I MHC, with a p53 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-08-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.330, 79.733, 85.849 |
Unit cell angles | 90.00, 101.68, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.370 |
R-factor | 0.2201 |
Rwork | 0.217 |
R-free | 0.27410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d2l |
RMSD bond length | 0.011 |
RMSD bond angle | 1.956 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.800 | 2.460 |
High resolution limit [Å] | 2.370 | 2.370 |
Rmerge | 0.109 | 0.566 |
Number of reflections | 38367 | 3805 |
<I/σ(I)> | 12 | |
Completeness [%] | 99.7 | |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 296 | 15% PEG 8000, 0.1 M Tris-HCl, 0.2 M magnesium chloride |