6VR0

Agrobacterium Tumefaciens ADP-glucose pyrophosphorylase W106A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9790
Spacegroup name	C 1 2 1
Unit cell lengths	433.318, 141.040, 92.510
Unit cell angles	90.00, 102.30, 90.00

Refinement procedure

Resolution	50.632 - 2.200
R-factor	0.2305
Rwork	0.214
R-free	0.24340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w6j
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.632	2.400
High resolution limit [Å]	2.200	2.200
Rmerge	0.125	0.948
Number of reflections	272607	13264
<I/σ(I)>	4.5
Completeness [%]	99.3
Redundancy	3.5
CC(1/2)	0.993	0.549

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	50mM HEPES pH 7.5 and 1.5 M Lithium sulfate