6VQE
HLA-B*27:05 presenting an HIV-1 13mer peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 105 |
| Detector technology | PIXEL |
| Collection date | 2010-09-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.940, 82.782, 109.426 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.181 - 1.770 |
| R-factor | 0.1625 |
| Rwork | 0.161 |
| R-free | 0.18610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w0v |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.181 | 46.180 | 1.810 |
| High resolution limit [Å] | 1.770 | 9.030 | 1.770 |
| Rmerge | 0.069 | 0.038 | 0.447 |
| Number of reflections | 45852 | 421 | 2489 |
| <I/σ(I)> | 13.7 | ||
| Completeness [%] | 99.8 | 99.7 | 96.7 |
| Redundancy | 6.1 | 5.5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 277 | 20 %w/v Polyethylene Glycol 3350, 0.2 M Potassium di-Hydrogen Phosphate |
