6VPW
1.90 Angstrom Resolution Crystal Structure Chemotaxis protein CheX from Vibrio vulnificus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-07-24 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 41 |
Unit cell lengths | 44.093, 44.093, 143.062 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.580 - 1.900 |
R-factor | 0.1766 |
Rwork | 0.174 |
R-free | 0.22530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3h2d |
RMSD bond length | 0.006 |
RMSD bond angle | 1.333 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.056 | 0.791 |
Rmeas | 0.063 | 0.887 |
Rpim | 0.028 | 0.397 |
Number of reflections | 21375 | 1065 |
<I/σ(I)> | 25 | 2.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5 | 4.8 |
CC(1/2) | 0.823 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | Protein: 10.4 mg/ml, 0.5M Sodium chloride, 0.01M Tris pH 8.3, Screen: Classics II (D7), 0.1M Bis-Tris pH 6.5, 25% (w/v) PEG 3350 |